CRISPR Primer expands with Type II sections

 
Blue Cas9 complex on DNA, with green protospacer on top DNA strand bound to the green region of crRNA, leaving open region in DNA where the lower strand is unbound. Each DNA strand has a “cut” gap at same distance from the PAM.
 

Our CRISPR Primer now covers Type II CRISPR systems!

When we launched our CRISPR Primer last fall we focused on Type I and III systems, the subject of much of our lab’s current research. For our first expansion of the Primer, we’ve added information about Type II systems, also known as CRISPR-Cas9 systems.

Type II systems aren’t as common in nature as Type I and III systems, but are more widely known – the relative simplicity of these systems, which have just one protein subunit, Cas9, made them easy to use for genome editing. Early Cas9 tools were the basis of the CRISPR revolution, including the Nobel Prize-winning work by Emmanuelle Charpentier and Jennifer Doudna. And now you can learn about Type II Interference and Type II Tools on our primer.

This isn’t the last expansion to our primer – keep an eye on our news posts and the CRIPSR Primer for more updates to come!

The CRISPR Primer is supported by funding from the Walder Foundation.

Sarah Studerprimer